|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Journal of Experimental Biology, Vol 174, Issue 1 65-80, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
SHP. Maddrell, W. S. Herman and J. A. Farndale RW andRiegel
Forskolin stimulates rapid fluid secretion by the Malpighian tubules of Rhodnius prolixus at concentrations above 5x10-6 mol l-1. In the presence of a threshold concentration of forskolin, the tubules are 30-50 times more sensitive to 5-hydroxytryptamine (5-HT) than in its absence. Similar synergism is seen between 5-HT and extracts of the mesothoracic ganglionic mass (which is rich in the peptide diuretic hormone, DH) and between 5-HT and samples of haemolymph, also rich in peptide DH, from fed insects 1-2 h after feeding. The dose-response curves for mixtures of forskolin and 5-HT and of peptide DH and 5-HT are all very steep, approximately five times steeper than for any one stimulant alone. Forskolin, 5-HT and extracts of the ganglionic mass all stimulated adenylate cyclase from broken membrane preparations from the Malpighian tubules in a dose-dependent manner and at doses similar to those required to stimulate fluid secretion by intact tubules. Mixtures of ganglionic extract and 5- HT stimulated adenylate cyclase activity in a synergistic fashion. Injections into fifth-instar Rhodnius, 24 h before feeding, of 5,7-dihydroxytryptamine, which is known to block or reduce 5-HT release, caused delays in the onset of the consequent diuresis or prevented it altogether. This is consistent with the proposal that the rapid onset of diuresis after feeding is caused by the simultaneous release of 5-HT and peptide DH acting synergistically.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  T. M. Clark, J. L. Lawecki, J. J. Shepherd, A. N. Hirschler, and T. R. Samandu Effects of serotonergic agents on survival and hemolymph composition of the larval mosquito Aedes aegypti (Diptera: Culicidae, L.) in vivo: does serotonin regulate hemolymph acid-base homeostasis? J. Exp. Biol., November 15, 2009; 212(22): 3728 - 3736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Coast Neuroendocrine control of ionic homeostasis in blood-sucking insects J. Exp. Biol., February 1, 2009; 212(3): 378 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Maddrell Insect homeostasis: past and future J. Exp. Biol., February 1, 2009; 212(3): 446 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. T. Brugge, D. A. Schooley, and I. Orchard Amino acid sequence and biological activity of a calcitonin-like diuretic hormone (DH31) from Rhodnius prolixus J. Exp. Biol., February 1, 2008; 211(3): 382 - 390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-P. Paluzzi and I. Orchard Distribution, activity and evidence for the release of an anti-diuretic peptide in the kissing bug Rhodnius prolixus J. Exp. Biol., March 1, 2006; 209(5): 907 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. V. Skaer, D. R. Nassel, S. H. P. Maddrell, and N. J. Tublitz Neurochemical fine tuning of a peripheral tissue: peptidergic and aminergic regulation of fluid secretion by Malpighian tubules in the tobacco hawkmoth M. sexta J. Exp. Biol., July 1, 2002; 205(13): 1869 - 1880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. I. M. Wiehart, S. W. Nicolson, R. A. Eigenheer, and D. A. Schooley Antagonistic control of fluid secretion by the Malpighian tubules of Tenebrio molitor: effects of diuretic and antidiuretic peptides and their second messengers J. Exp. Biol., February 15, 2002; 205(4): 493 - 501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Te Brugge, S. Miksys, G. Coast, D. Schooley, and I Orchard The distribution of a CRF-like diuretic peptide in the blood-feeding bug Rhodnius prolixus J. Exp. Biol., January 8, 1999; 202(15): 2017 - 2027. [Abstract] [PDF]
|
|
