|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
Journal of Experimental Biology, Vol 171, Issue 1 15-42, Copyright © 1992 by Company of Biologists
JOURNAL ARTICLES |
JH Hayashi and RB Levine
Division of Neurobiology, University of Arizona, Tucson 85721.
During insect metamorphosis the nervous system is reorganized to accommodate changes in behavior. In the hawkmoth, Manduca sexta, many identified larval motoneurons persist to innervate new adult muscles, while undergoing changes in dendritic morphology and synaptic connections. The thoracic leg motoneurons, for example, innervate different sets of muscles in the larva and adult and participate in distinct types of locomotor behavior in the two stages of life. To determine whether changes in the biophysical properties of these motoneurons accompany the structural and functional modifications that have been described, we used the whole-cell voltage-clamp technique to compare the Ca2+ and K+ currents expressed by leg motoneurons isolated from the larval, pupal and adult stages of Manduca. After 24 h in culture, the somata of leg motoneurons isolated from all three stages expressed voltage-sensitive Ca2+ currents that could be blocked by Cd2+, Co2+ or Ni2+. The currents were larger with Ba2+ as the charge carrier. The Ca2+ current density was significantly lower in these motoneurons during the early pupal stage than in either the larva or adult. Similar experiments revealed both transient and sustained K+ currents in the leg motoneurons that could be blocked with Cs+. There was a significant decrease in the density of the transient, inactivating outward current in leg motoneurons isolated from the early pupal stage. Thus, the levels of some types of ionic currents are modulated during metamorphosis. These changes may be important for the developmental or behavioral changes that accompany metamorphosis.
This article has been cited by other articles:
|
|
 |
|
  A. Husch, S. Hess, and P. Kloppenburg Functional Parameters of Voltage-Activated Ca2+ Currents From Olfactory Interneurons in the Antennal Lobe of Periplaneta americana J Neurophysiol, January 1, 2008; 99(1): 320 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Keyser and J. L. Witten Calcium-activated potassium channel of the tobacco hornworm, Manduca sexta: molecular characterization and expression analysis J. Exp. Biol., November 1, 2005; 208(21): 4167 - 4179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Mercer, P. Kloppenburg, and J. G. Hildebrand Plateau Potentials in Developing Antennal-Lobe Neurons of the Moth, Manduca sexta J Neurophysiol, April 1, 2005; 93(4): 1949 - 1958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Wustenberg, M. Boytcheva, B. Grunewald, J. H. Byrne, R. Menzel, and D. A. Baxter Current- and Voltage-Clamp Recordings and Computer Simulations of Kenyon Cells in the Honeybee J Neurophysiol, October 1, 2004; 92(4): 2589 - 2603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Grunewald Differential expression of voltage-sensitive K+ and Ca2+ currents in neurons of the honeybee olfactory pathway J. Exp. Biol., January 1, 2003; 206(1): 117 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Mercer and J. G. Hildebrand Developmental Changes in the Density of Ionic Currents in Antennal-Lobe Neurons of the Sphinx Moth, Manduca sexta J Neurophysiol, June 1, 2002; 87(6): 2664 - 2675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Goriely, T. W. Secomb, and L. P. Tolbert Effect of the Glial Envelope on Extracellular K+ Diffusion in Olfactory Glomeruli J Neurophysiol, April 1, 2002; 87(4): 1712 - 1722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Duch and R. B. Levine Changes in Calcium Signaling During Postembryonic Dendritic Growth in Manduca sexta J Neurophysiol, March 1, 2002; 87(3): 1415 - 1425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Duch and R. B. Levine Remodeling of Membrane Properties and Dendritic Architecture Accompanies the Postembryonic Conversion of a Slow into a Fast Motoneuron J. Neurosci., September 15, 2000; 20(18): 6950 - 6961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Haag and A. Borst Spatial Distribution and Characteristics of Voltage-Gated Calcium Signals Within Visual Interneurons J Neurophysiol, February 1, 2000; 83(2): 1039 - 1051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Lee, Y. Park, T. M. Brown, and M. E. Adams Altered Properties of Neuronal Sodium Channels Associated with Genetic Resistance to Pyrethroids Mol. Pharmacol., March 1, 1999; 55(3): 584 - 593. [Abstract] [Full Text]
|
|
|
|
|
|
R. Hewes Voltage-dependent ionic currents in the ventromedial eclosion hormone neurons of Manduca sexta J. Exp. Biol., January 9, 1999; 202(17): 2371 - 2383. [Abstract] [PDF]
|
|
|
|
|
|
C Benkenstein, M Schmidt, and M Gewecke Voltage-activated whole-cell K+ currents in lamina cells of the desert locust schistocerca gregaria J. Exp. Biol., January 7, 1999; 202(14): 1939 - 1951. [Abstract] [PDF]
|
|
|
|
|
|
P. Kloppenburg, B. S. Kirchhof, and A. R. Mercer Voltage-Activated Currents From Adult Honeybee (Apis mellifera) Antennal Motor Neurons Recorded In Vitro and In Situ J Neurophysiol, January 1, 1999; 81(1): 39 - 48. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Mills and R. M. Pitman Electrical Properties of a Cockroach Motor Neuron Soma Depend on Different Characteristics of Individual Ca Components J Neurophysiol, November 1, 1997; 78(5): 2455 - 2466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wicher and H. Penzlin Ca2+ Currents in Central Insect Neurons: Electrophysiological and Pharmacological Properties J Neurophysiol, January 1, 1997; 77(1): 186 - 199. [Abstract] [Full Text] [PDF]
|
|
