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Journal of Experimental Biology 168,93-110 (1992)
Published by Company of Biologists 1992

Allosteric Modulation of Haemocyanin Oxygen-Affinity by l-Lactate and Urate in the Lobster Homarus Vulgaris : I. Specific and Additive Effects on Haemocyanin Oxygen-affinity

B. ZEIS 1, A. NIES 1, C. R. BRIDGES 1, and M. K. GRIESHABER 1

1 Institut für Zoologie, Lehrstuhl für Tierphysiologie, Heinrich-Heine-Universität Universitätsstra{beta}ie 1, D-4000 Düsseldorf 1, Germany

The specific effects of L-lactate and urate on oxygen binding by the haemocyanin of the lobster Homarus vulgaris were investigated. Increasing concentrations of l-lactate were found to increase haemocyanin oxygen-affinity. The relationship between the oxygen affinity ({Delta}logP50) and [l-lactate] expressed as ({Delta}logP50)({Delta}log[l-lactate])-1 was -0.11 at l-lactate concentrations between 0.3 and 11mmoll-1 and pH7.99±0.03. Urate, likewise, had a potentiating effect on haemocyanin oxygen-affinity: ({Delta}logP50)({Delta}log[l-lactate)-1 was -0.18 at urate concentrations between 0 and 0.93mmoll-1 and pH7.99±0.03. Cooperativity, expressed as n50, was reduced by the presence of both modulators.

The influence of the simultaneous presence of both factors on haemocyanin oxygen-affinity was also investigated. The effects of l-lactate and urate on haemocyanin oxygen-affinity were found to be additive. The possible physiological role of these modulators is discussed.

Note:
To whom reprint requests should be addessed.

Key words: lobster haemocyanin, oxygen equilibrium curve, l-lactate, urate, cooperativity, Homarus vulgaris.

Accepted on March 17, 1992




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© The Company of Biologists Ltd 1992