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Journal of Experimental Biology 150,81-93 (1990)
Published by Company of Biologists 1990

The Intracellular _pH of a Molluscan Smooth Muscle During a Contraction-Catch-Relaxation Cycle Estimated by the Distribution of [¹⁴C]DMO and by ³¹P-NMR Spectroscopy

J. ZANGE ¹, H. O. PÖRTNER ¹, A. W. JANS ², and M. K. GREESHABER ¹

¹ Zoologisches Institut, Lehrstuhl fr Tierphysiologie, Heinrich-Heine-Universität Universitätsstrasse 1, 4000 Düsseldorf, FRG
² Max-Planck-Institut für Systemphysiologie, Rheinlanddamm 201, 4600 Dortmund, FRG

The intracellular pH (pHi) of the anterior byssus retractor muscle (ABRM) of Mytilus edulis L. was estimated during rest and during a contraction-catch-relaxation cycle by the distribution of [¹⁴C]DMO and by ³¹P-NMR spectroscopy. The pHi of resting muscles was found to be nearly the same whether it was determined with DMO (7.41±0.06, N=5l) or by ³¹P-NMR spectroscopy (7.44±0.02, N=5). During catch the pHi was not significantly different from its value at rest. Serotonin (10^-5moll^-1) induced a rapid relaxation and a significant increase in pHi (DMO: 7.56±0.05, N=5; ³¹P-NMR: 7.57±0.02, N=5). In resting ABRM serotonin (10^-6moll^-1) also induced an alkalosis. It was shown that cyclic AMP, the second messenger of serotonin, elicited an increase of pHi. Dopamine (10^-5moll^-1) did not cause an increase in either cyclic AMP levels or pHi. The rates by which tonically contracted ABRM loaded at 0.1 N were stretched were significantly increased by an alkalosis elicited by the addition of 10 or 20mmoll^-1 methylamine. Acidosis elicited by lO mmoll^-1 DMO caused a significant decrease in the rate of relaxation.

Key words: ABRM, catch, intracellular _pH, serotonin, cyclic AMP

Accepted on December 13, 1989

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