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Journal of Experimental Biology 149,425-437 (1990)
Published by Company of Biologists 1990

INFLUENCE OF ORGANIC PHOSPHATES ON THE ROOT EFFECT OF MULTIPLE FISH HAEMOGLOBINS

BERND PELSTER ¹ and ROY E. WEBER ²

¹ 1Institut für Physiologie, Ruhr-Universitat Bochum, FRG
² Zoophysiology Laboratory, Aarhus University Denmark

The influence of organic phosphates on the reduction in oxygen-carrying capacity at low pH (Root effect) in multiple fish haemoglobins has been analysed spectrophotometrically. In stripped haemolysates of carp, trout and eel, the Root effect in the presence of ATP was manifested below pH7.0. In the absence of phosphates, it was only found in trout haemolysate

In the pH range between 8.5 and 6.1 no Root effect could be induced in the cathodic component (Hbl) of either trout or eel haemoglobin, even in the presence of very high concentrations of ATP or GTP. This was also true for component II (Hbll) of trout. The anodic component (HblV) of both species, however, exhibited a strong Root effect potentiated by NTP. At the same NTP/Hb₄ concentration ratio, GTP was much more effective than ATP in both species

The involvement of different haemoglobin components in the generation of high oxygen tensions in the fish swimbladder is discussed by comparing in vivo Root effect data obtained with an eel swimbladder preparation with in vitro data measured in eel blood and haemoglobin.

Key words: haemoglobin, Root effect, organic phosphates

Accepted on November 15, 1989

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