QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by BENKE, D. Articles by BREER, H. Search for Related Content PubMed Articles by BENKE, D. Articles by BREER, H.

Journal of Experimental Biology 147,329-342 (1989)
Published by Company of Biologists 1989

Monoclonal Antibodies Detecting Regulatory Polypepttdes of the Insect Neuronal Acetylcholine Receptor

D. BENKE ¹, I. STAHMER ², and H. BREER ³

¹ University of Hohenheim, Institute of Zoophysiology 7000 Stuttgart, West Germany; Present address: Institut für Molekulare Immunologie, Parkallee 2a, 2061 Borstel, West Germany
² University of Hohenheim, Institute of Zoophysiology 7000 Stuttgart, West Germany;
³ University of Hohenheim, Institute of Zoophysiology 7000 Stuttgart, West Germany

Please address reprint requests to Professor H. Breer, Universität Stuttgart, Hohenheim Farbenstrasse 30, 7000 Stuttgart 70

1. Monoclonal antibodies affecting the ligand binding of the neuronal acetylcholine receptor were prepared.

2. A clone was detected which produced antibodies that increased [¹²⁵I]-bungarotoxin binding, but decreased [³H]acetylcholine binding.

3. Western blot analysis established that these antibodies did not recognize the receptor protein, but labelled a 20xl0³M_r polypeptide.

4. This putative regulatory polypeptide was purified and was found to inhibit [¹²⁵I]--bungarotoxin binding to pretreated neuronal membranes.

Key words: acetylcholine receptor, antibodies, polypeptides, insect.

Accepted on July 11, 1989