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Journal of Experimental Biology 144,463-478 (1989)
Published by Company of Biologists 1989

The Seasonal Modulation of Na⁺/H⁺ Exchanger Activity in Trout Erythrocytes

ANDREW R. COSSINS ¹ and ROBERT V. KILBEY ¹

¹ Department of Evolutionary and Environmental Biology, University of Liverpool, PO Box 147, Liverpool L69 3BX

A pH-stat method is described for determining the initial rate of efflux of H⁺ equivalents from trout erythrocytes after stimulation with catecholamines. This measure of transport capacity of the Na⁺/H⁺ exchanger was sensitive to medium pH with a peak at 7.24 ± 0.09 pH units. After washing blood into a trout saline the exchange capacity recovered to a maximal value after 2 h and remained stable for at least 24 h at 5°C. Internal Na⁺ concentration reached a low, stable value also within 2-3 h of washing. The antiporter was comparatively unstable when incubated at room temperature. The concentrations for half-maximal responses to the agonists adrenaline, noradrenaline and isoproterenol were 3.0 ± 1.1 x 10^-7, 2.5 ± 3.0 x 10^-8 and 1.5 ± 0.6 x 10^-8mol l^-1, respectively.

The variation of antiporter capacity with time of the year was followed over a 24month period. Capacity was highest during early summer at 200-250 mmol h^-1 l^-1 packed cell volume (pcv), but declined from November to March to a low of approximately 100 mmol h^-1 l^-1 pcv. Exchange capacity increased rapidly during the early spring back to values characteristic of early summer. It appears that both temperature and photoperiod are involved in regulating the spring increase.

Key words: Na⁺/H⁺ exchange, adrenergic responses, rainbow trout, erythrocytes, season

Accepted on March 8, 1989

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