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Journal of Experimental Biology 137,319-339 (1988)
Published by Company of Biologists 1988

Modulation of Ionic Currents by Synaptic Action and 5-HT Application in the Identified Heart Excitatory Neurone of the African Giant Snail, Achatina Fulica Férussac

Yasuo Furukawa 1 and Makoto Kobayashi 1

1 Physiological Laboratory, Faculty of Integrated Arts and Sciences, Hiroshima University, Hiroshima, Japan

In the African giant snail, Achatina fulica Férussac, the ionic mechanisms underlying slow depolarization of a heart excitatory neurone, PON, induced by the activity of two cerebral ganglionic neurones, d-RCDN and d-LCDN, were investigated under voltage-clamp.

The slow depolarization of PON that was induced by the activity of the cerebral neurones was blocked by the serotonin (5-HT) antagonist, methysergide. Bath application of 5-HT to the axotomized PON produced a similar slow depolarization, which was also blocked by methysergide. These results suggest that the neurotransmitter of d-RCDN and d-LCDN is 5-HT.

Under voltage-clamp, activity of the cerebral neurones usually produced an inward shift in the holding current of PON with a decrease of conductance. Ionic substitution experiments and injection of Cs+ into PON showed that the response was mainly due to a decrease in K+ conductance. In some cases, this inward shift showed two components: an early component with increased conductance and a late one with decreased conductance. The early component was not decreased by Cs+ injection but was augmented by EGTA injection into PON, which may suggest the involvement of a Ca2+ conductance in this synaptic response.

Application of 5-HT produced a similar inward shift in holding current which was also mainly the result of a decrease in the background K+ current. 5-HT was also found to increase the voltage-dependent Ca2+ current and the inward rectifying K+ current.

The significance of these results is discussed in relation to the heart regulation of this snail.

Key words: snail neurone, modulation of ionic current, serotonin, voltage-clamp.

Accepted on November 25, 1987






© The Company of Biologists Ltd 1988