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Journal of Experimental Biology 136,23-34 (1988)
Published by Company of Biologists 1988

Effects of Ions on Nematocysts Isolated from Acontia of the Sea Anemone Calliactis Tricolor by Different Methods

MICHIO HIDAKA 1 and RICHARD N. MARISCAL 2

1 Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA; Department of Biology, University of the Ryukyus, Nishihara, Okinawa 903-01, Japan
2 Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA

Differences in nematocyst isolating procedures significantly affected the discharge characteristics of the acontial nematocysts isolated from Calliactis tricolor.

Most of the nematocysts isolated in artificial sea water (ASW) discharged instantaneously in 5 mmol l-1 EGTA and to a lesser degree in distilled water (DW), but they did not discharge in Ca2+-free ASW or 50 mmol l-1 CaCl2.

Most of the nematocysts isolated in 1 mol l-1 sodium citrate discharged instantaneously when treated with any of the above solutions.

Nematocysts isolated in DW or 1 mol l-1 sucrose exhibited a modest degree of discharge in 5 mmol l-1 EGTA after a considerable latent period.

Some of the present results appear to be inconsistent with the hypothesis that removal of Ca2+ from the intracapsular fluid causes nematocyst discharge.

Key words: nematocyst, sea anemone, calcium

Accepted on November 25, 1987






© The Company of Biologists Ltd 1988