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Journal of Experimental Biology 117,119-132 (1985)
Published by Company of Biologists 1985

An Investigation of Haemocyanin Oxygen Affinity in the Semi-Terrestrial Crab Ocypode Saratan Forsk

S. MORRIS 1 and C. R. BRIDGES 1

1 Institut für Zoologie IV, Lehrstuhl für Stoffwechselphysiologie, Universität Düsseldorf D-4000 Düsseldorf, F.R.G

The oxygen affinity of the haemocyanin in the supralittoral crab Ocypode saratan was investigated at temperatures between 20 and 35 °C. The effect of L-lactate on dialysed and undialysed haemolymph oxygen affinity was also examined.

In general, the temperature sensitivity of the haemocyanin was low: {delta}H was –3.1kJ mol-1, between 25 and 30°C. Temperature sensitivity was temperature-dependent, being larger at the extreme temperatures ({Delta}H = –26kJ mol-1). The Bohr effect ({Delta}logP50/{Delta}pH) was temperature-independent and averaged – 0.67.

No specific effect of CO2 on oxygen affinity was observed but L-lactate increased oxygen affinity in both dialysed and undialysed haemolymph. The maximal effect of lactate on oxygen affinity was similar in dialysed and undialysed haemolymph, but was evident at a lower lactate concentration (4mmol1-1) in dialysed, compared with undialysed, haemolymph (7mmoll-1). Dialysed haemolymph showed a higher oxygen affinity than undialysed haemolymph at low lactate concentration (<4mmoll-1). The Bohr effect and buffer value both decreased with increasing lactate concentration in both dialysed and undialysed haemolymph. The physiological implications of these findings are discussed.

Key words: Land crabs, oxygen affinity, haemocyanin, temperature effect, lactate effect

Submitted on December 21, 1984


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© The Company of Biologists Ltd 1985