spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Klein, M. G.
Right arrow Articles by Prosser, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Klein, M. G.
Right arrow Articles by Prosser, C. L.

Journal of Experimental Biology, Vol 114, Issue 1 563-579, Copyright © 1985 by Company of Biologists

JOURNAL ARTICLES

The effects of temperature acclimation on the resting membrane of skeletal muscle fibres from green sunfish

MG Klein and CL Prosser

Conductive properties of muscle fibres from green sunfish (Lepomis cyanellus) acclimated to different temperatures were examined. The relative membrane permeability to chloride and potassium ions, PCl/PK, measured at acclimation temperature, was approximately 7.0 after acclimation at 25 degrees C and 1.3 after acclimation at 7 degrees C. This difference was due to a six-fold reduction in the membrane chloride conductance upon acclimation to 7 degrees C as compared to 25 degrees C-acclimated fibres. Mean (+/- S.E.M.) values of the chloride conductance were 554 +/- 68 microseconds cm-2 in warm-acclimated sunfish, and 75 +/- 9 microseconds cm-2 in cold-acclimated sunfish. Membrane capacitance also differed significantly between the two acclimation groups. When the temperature was varied acutely, the magnitude of the chloride conductance exhibited a maximum Q10 of only 1.9, compared with a Q10 of 3.0 associated with acclimation. Upon transferring 25 degrees C-acclimated sunfish to holding tanks at 7 degrees C, the total membrane resistance exhibited a sigmoidal increase over about 14 days, and a steady membrane capacitance was achieved in about 10 days. For 7 degrees C-acclimated sunfish, transferred to 25 degrees C, resistance showed a sigmoidal decrease over 10 days and capacitance was steady after 8 days. The results indicate that thermal acclimation of the muscle membrane involves cellular regulatory processes which underlie significant changes in the electrical properties of the fibre.




© The Company of Biologists Ltd 1985