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Journal of Experimental Biology 107,473-489 (1983)
Published by Company of Biologists 1983

-Bungarotoxin Blocks Excitatory Synaptic Transmission between Cercal Sensory Neurones and Giant Interneurone 2 of the Cockroach, Peripianeta Americana

D.B. SATTELLE ¹, I.D. HARROW ², B. HUE ³, M. PELHATE ⁴, J.I. GEPNER ⁵, and L.M. HALL ⁵

¹ A.R.C. Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, Cambridge University Downing Street, Cambridge, CB2 3EJ
² Department of Biological Sciences, Sherman Fairchild Center for the Life Sciences, Columbia University New York, N.Y. 10027, U.S.A.; A.R.C. Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, Cambridge University Downing Street, Cambridge, CB2 3EJ
³ Laboratoire de Physiologie, Faculté de Médecine, Université d'Angers, Angers, 49045, France
⁴ Laboratoire de Physiologie, Faculté de Médecine, Université d'Angers Angers, 49045, France
⁵ Department of Genetics, Albert Einstein College of Medicine 1300 Morris Park Avenue, Bronx, N.Y. 10461, U.SA.

To whom correspondence concerning this manuscript should be addressed.

1. Autoradiographic localization of an ¹²⁵I--bungarotoxin binding component revealed that specific binding was distributed mainly in the neuropile and to some extent in the periphery of the terminal abdominal ganglion of the cockroach, Periplaneta americana (L.).

2. Action potentials recorded from the axon of GI2 under current-clamp conditions were not affected by exposure to 1.0x10^-5M -bungarotoxin.

3. Excitatory postsynaptic potentials recorded from GI2, evoked by stimulation of cereal sensory neurones, were sensitive to block by relatively low concentrations (1.0x10^-9–1.0x10^-7 M) of -bungarotoxin. The time-course for blockade was found to depend on toxin concentration and frequency of afferent stimulation.

4. Excitatory postsynaptic potentials recorded from GI 2 were not affected by exposure to 1.0x10^-6M quinuclidinyl benzilate.

5. We conclude that at least a portion of the ¹²⁵I--bungarotoxin binding component represents cholinergic receptors which have a postsynaptic function at synapses between cereal sensory neurones and GI 2.

Key words: -Bungarotoxin, Periplaneta americana CNS, synaptic transmission

Submitted on March 23, 1983
Accepted on May 25, 1983

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