spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by PRIMOR, N.
Right arrow Search for Related Content
PubMed
Right arrow Articles by PRIMOR, N.
Journal of Experimental Biology 105,83-94 (1983)
Published by Company of Biologists 1983

Pardaxin Produces Sodium Influx in the Teleost Gill-like Opercular Epithelia

NAFTALI PRIMOR 1

1 Department of Physiology and Biophysics, New York University Medical Center, 550 First Avenue, New York, New York 10016, U.S.A. and Osborn Laboratories of Marine Sciences, New York Aquarium, New York Zoological Society, Brooklyn, New York 11224, U.S.A.; Osborn Laboratories of Marine Sciences, New York Aquarium, New York Zoological Society, Brooklyn, New York 11224 U.S.A.

1. Transport by the gill-like opercular epithelium of the teleost, Fundulus heteroclitus, was affected by pardaxin, a protein that is toxic to fish, isolated from the Red Sea flatfish Pardachirus marmoratus.

2. Administration of pardaxin to the mucosal (seawater) side of the isolated short-circuited opercular epithelium, caused a transient stimulation of the active transport of ions (Isc), followed by an inhibition. The Isc stimulation was abolished by ouabain or/and in Na+-free Ringer but not in Cl--or HCO3--Ringer. When applied to the serosal (blood) side, pardaxin did not affect the Isc.

3. Pardaxin produced a net transient Na+ current from the mucosal side to the serosal side of 2.2 µequiv cm-2h-1. It is concluded that this Na+influx caused the Isc stimulation. The influx is suggested to be the mechanism of pardaxin's toxicity in fish.

Key words: Pardaxin, fish toxin, ion-osmoregulation, skin epithelium.

Submitted on August 16, 1982
Accepted on January 31, 1983






© The Company of Biologists Ltd 1983