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Journal of Experimental Biology 105,339-350 (1983)
Published by Company of Biologists 1983

Acetylcholine Receptors on the Cell Body Membrane of Giant Interneurone 2 in the Cockroach, Periplaneta Americana

I. D. HARROW 1 and D. B. SATTELLE 2

1 Department of Zoology, University of Cambridge, Downing Street, Cambridge, U.K.; Department of Biological Sciences, Columbia University, New York, N.Y., 10027, U.S.A.
2 A.R.C. Unit of Insect Neurophysiology and Pharmacology, Department of Zoology Downing Street, Cambridge, U.K.

1. Ionophoresis of acetylcholine (ACh) onto the cell body membrane of an identified giant interneurone (GI2) in the central nervous system of the cockroach Periplaneta americana induced a depolarizing response at resting potential which was attributed to a population of extrasynaptic ACh receptors. 2. The sensitivity of the cell body membrane of GI 2 to ionophoresis of ACh was determined. 3. Perfusion of 1.0 x 10-6M neostigmine, an inhibitor of acetylcholinesterase, potentiated the ACh sensitivity of the cell body membrane of GI2. This indicated that a high acetylcholinesterase activity was present in the periphery of the sixth abdominal ganglion (A6). 4. The nicotinic antagonist, {alpha}-bungarotoxin (at a concentration of 1.0 x 10-7M) was found to block the ACh response of the cell body membrane of GI2. By contrast, the muscarinic antagonist, quinuclidinyl benzilate, (at concentrations up to 1.0 x 10-5 M) had no detectable effect on the ACh response. 5. It is suggested that an extrasynaptic nicotinic type of ACh receptor is present on the cell body membrane of GI2.

Key words: Acetylcholine receptors, interneurone, cockroach

Submitted on November 29, 1982
Accepted on February 16, 1983






© The Company of Biologists Ltd 1983