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Journal of Experimental Biology, Vol 103, Issue 1 95-108, Copyright © 1983 by Company of Biologists


JOURNAL ARTICLES

Oxygenational properties and phosphorylated metabolic intermediates in blood and erythrocytes of the dogfish, Squalus acanthias

RM Wells and RE Weber

A typical whole blood O2-equilibrium curve from Squalus acanthias had a P50 of 13.2 mmHg and was slightly sigmoidal, having an n value of 1.6 at 15 degrees C, PCO2 = 2.2 mmHg (pH = 7.85). A small Bohr effect was present (phi = -0.28) together with a weak Haldane effect and no Root shift. The predominant trinucleotide, determined by thin layer chromatography, was ATP (0.44 +/- 0.13 S.D. mmol 1(-1) blood) with smaller amounts of GTP present (0.07 +/- 0.02) S.D. mmol 1(-1). Total nucleotide concentrations, determined enzymatically, were low by comparison with teleosts. Incubation of erythrocytes with or without oxygen, or in the presence of a metabolite-enriched 'cocktail' showed limited potential for phosphate cofactor regulation of blood oxygen affinity.


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C. Larsen, H. Malte, and R. E. Weber
ATP-induced Reverse Temperature Effect in Isohemoglobins from the Endothermic Porbeagle Shark (Lamna nasus)
J. Biol. Chem., August 15, 2003; 278(33): 30741 - 30747.
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© The Company of Biologists Ltd 1983