Fig. 4. Confocal microscopic and western blot analyses of Xenopus oocytes injected with turtle UT cRNA. (A) Immunofluorescence images for turtle UT protein in turtle UT cRNA-injected oocytes using affinity-purified turtle UT antibody. Immunoreactive proteins were detected in the plasma membrane (arrowheads) of turtle cRNA-injected oocytes (a). The protein was not detected in the plasma membrane (arrowheads) of water-injected oocytes (b). Scale bars, 500 µm. (B) Western blot analysis of the membrane fractions prepared from the turtle UT-injected Xenopus oocytes, and those from the desiccated turtle kidney, heart and liver using the anti-turtle UT antibody. An immunoreactive band was detected at an approximately 58 kDa in extract of the UT-injected oocytes (lane 1) and no band was observed in the water-injected oocytes (lane 2). The 58 kDa band was detected in extract of the kidney (lane 3). No band was observed in either heart (lane 4) or liver (lane 5).