Fig. 2. Tissue-specific expression of turtle UT mRNA determined using RT-PCR. PCR was performed using specific primers for turtle UT and turtle glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Turtle UT mRNA was expressed in kidney but was not detected in the other tissues examined. A smaller band that was shown by sequencing to be non-specific was found in the stomach. In this experiment the expression level of turtle UT was normalized by RT-PCR amplification of the same cDNA templates using the GAPDH primer.