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Figure 7


Fig. 7. Validation of probe specificity. Pair wise, adjacent FW gill sections were probed with AP-labelled {alpha}1a (A,B) or {alpha}1b (C,D) cDNA probes either alone (A,C) or in combination with 10-fold excess of the corresponding unlabelled cDNA sequence of the alternate isoform (i.e. {alpha}1b in B and {alpha}1a in D). No significant displacement of labelled probe takes place in either experiment, showing that the two probes are specific for their respective mRNA targets. (E,F) The sections were probed with the AP-labelled {alpha}1b probe either alone, E, or in combination with 5-fold excess of the corresponding unlabelled {alpha}1b cDNA sequence, F. The labelled probe was fully displaced under these conditions. Bars indicate 100 µm. AP, alkaline phosphatase; FW, freshwater.