Fig. 3. RT-PCR analysis of (A) PGC-1
, (B) PPAR
and (C) reptilian UCP
mRNA levels in muscle from controls, supercooled, frozen and thawed lizards
(Lacerta vivipara). For details, see Materials and methods.
β-actin was used as an invariant gene. Values are means ± s.e.m.
for eight lizards in each group. Different letters indicate a significant
difference (P<0.05).