Fig. 2. MALDI-FTMS identification of native Pugettia producta neuropeptides. (A–C) Representative direct tissue spectra of the (A) sinus gland (SG), (B) stomatogastric ganglion (STG) and (C) pericardial organ (PO). In the SG, a peak corresponding to the [M+Na]⁺ ion of pELNFSPGWamide (red pigment concentrating hormone; RPCH) was routinely detected. In the STG, the [M+H]⁺ ion corresponding to APSGFLGMRamide (authentic Cancer borealis tachykinin-related peptide I; CabTRP I) was present in most spectra. In PO samples, the [M+H]⁺ ion of PFCNAFTGCamide (crustacean cardioactive peptide; CCAP) was commonly seen. In addition, peaks corresponding to other peptides were also detected in these tissues, some of which are labeled in the spectra. m/z, mass/charge; Gly¹-SIFa, GYRKPPFNGSIFamide.