Fig. 2. Densities and distribution of DHPRs and RyRs in the skin of coeval adult female (non-layers) and male chickens on a low-Ca²⁺ diet. (A, B) Fluorescence intensities in different skin layers after incubation with high-affinity (–)-enantiomers of DHP and ryanodine, respectively. (C) Distribution of DHPRs in the skin of one female, non-laying chicken. (D) Distribution of DHPRs in the skin of one coeval male chicken. (E) Distribution of RyRs in the skin of one female, non-laying chicken. (F) Distribution of RyRs in the skin of one male chicken. Error bars denote s.e.m.; ST-SC, innermost SC and the interface between the ST and SC; EPI, mid-epidermis; D, outermost dermis. ^*P<0.05, post hoc tests. Bar, 50 µm.