Fig. 1. Densities and distribution of dihydropyridine receptors (DHPRs) and ryanodine receptors (RyRs) in the skin of coeval adult female (layers) and male chickens. (A) Fluorescence intensities (a.u., arbitrary units) in different skin layers after incubation with a high-affinity (–)-enantiomer of DHP. (B) Fluorescence intensities in different skin layers after incubation in high-affinity (–)-enantiomer of ryanodine. (C) Distribution of DHPRs in the skin of one laying female. (D) Distribution of DHPRs in the skin of one male chicken. (E) Distribution of RyRs in the skin of one laying female. (F) Distribution of RyRs in the skin of one male chicken. Error bars denote the standard error of the mean (s.e.m.); ST-SC, innermost stratum corneum (SC) and the interface between the stratum transitivum (ST) and SC; EPI, mid-epidermis; D, outermost dermis. ^**P<0.01, ^***P<0.001, post hoc tests. Bar, 50 µm.