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Figure 8


Fig. 8. Responses to adenosine 5'-[{gamma}-thio]-triphosphate (ATP{gamma}S) and {alpha}-β-methyleneadenosine 5'-triphosphate ({alpha}-β-MeATP) in stomach muscle preparations. (A) Concentration-dependent increase in mean force in response to ATP{gamma}S. A statistically significant increase was obtained at >=30 µmol l–1 in prometamorphic (N=7), at >=100 µmol l–1 (N=9) in metamorphic and at 300 µmol l–1 in juvenile tissues (N=15). (B) Concentration-dependent increase in mean force in response to {alpha}-β-MeATP. Statistically significant increase obtained at >=10 µmol l–1 in prometamorphic (N=8) and at >=30 µmol l–1 (N=6) in metamorphic and juvenile tissues (N=10). Control value prior to administration of agonist is denoted C on the x-axis. Statistical analysis was performed using repeated measures one-way ANOVA followed by Dunnett's post hoc test. (C) Effect of {alpha}-β-MeATP in the presence and absence of the P2 receptor antagonist pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS, 100 µmol l–1) in prometamorphic (10 µmol l–1 {alpha}-β-MeATP, N=7) and juvenile (30 µmol l–1 {alpha}-β-MeATP, N=8) stomach strips. Statistical analysis was performed using repeated measures one-way ANOVA followed by Bonferroni's post hoc test. Asterisk indicates P<0.05.