Fig. 4. Relative expression of CS mRNA and of the different COX subunit mRNAs in hepatocytes of cold- (white bars) and warm- (grey bars) acclimated eelpout (Z. viviparus). Relative amounts of CS (A–C), COX2 (D–F) and COX4 (G–I) mRNA were determined in freshly isolated hepatocytes (A,D,G; N=4) and in cells after incubation at either 4 or 11°C (B,C,E,F,H,I; N=5–8) under control conditions (white bars) or with the addition of adenosine (hatched bars). mRNA levels in equal amounts of total RNA were determined by RPA and normalized to the mean of freshly isolated hepatocytes from warm-acclimated fish. ^*Significant difference from control group incubated at the same temperature; significant difference from corresponding group incubated at 4°C. Values are means ± s.e.m.