Fig. 5. Double immunolabelling of serotonin (5-HT; green) and nerves (using a
neuronal marker zn12; red) in longitudinal sections (30 µm thick) of the
gill filament and lamellae of (A) O. mykiss, (B) C. auratus,
(C) H. lacerdae and (D) H. malabaricus. Images were captured
using a confocal scanning system. Inset in B shows close association of nerves
(red) and 5-HT-IR cell at the tip of the lamellae of C. auratus.