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Figure 4


Fig. 4. Expression of Seraf in trout embryo. (A) Stage 10B embryo (approximately 10 somites). Dorsal view (anterior to the top): labelling is observed in somites (arrowheads) as well as in differentiating notochord (arrow). (B) Stage 20 embryo (segmentation is complete). Lateral view: labelling is observed in somites and cephalic mesenchyme. (C,D) Stage 13 embryo (approximately 30 somites). Transverse sections through posterior (C) and anterior trunk (D) showing the progressive disappearance of Seraf transcript in the notochord and the medial part of the somite. (E) Eyed-stage embryo. Transverse section through the middle trunk. Seraf transcript is observed in the ventral and dorsal tips of the myotome (arrowheads), in external cells (arrows) and at the dorsal midline. Scale bar, 100 µm in A, 200 µm in B, 25 µm in C, and 30 µm in D and E.