Fig. 3. Expression of collectin subfamily member 12 in trout embryo. (A) Stage 10B embryo (approximately 10 somites). Dorsal view (anterior to the top): labelling is present in somites (arrow), lateral mesoderm and cephalic mesenchyme. (B) Stage 12 embryo (approximately 25 somites). Lateral view: labelling progresses caudally as somites form. (C) Eyed-stage embryo. Lateral view: labelling has progressed up to the most caudal somites. (D) Stage 10B embryo. Transverse section through the middle trunk: labelling is confined to a narrow dorsal domain of the somite (arrow). (E) Stage 20 somite. Transverse section through the anterior trunk: labelling extends to a large domain of the somite and is detectable in sclerotome cells (arrow). (F) Stage 35 somite embryo. Transverse section through the anterior trunk: labelling has narrowed to the external cells and is present in sclerotome cells surrounding the notochord. (G) Stage 20 embryo (segmentation is complete). Transverse section through the middle trunk: labelling is evident in the external cells (arrow), the facing epidermis (arrowhead) and in sclerotome cells. Scale bar, 150 µm in A, 200 µm in B, 300 µm in C, 20 µm in D, 40 µm in E and F, and 50 µm in G.