Fig. 5. The survival of frozen salivary glands was unaffected by the presence of an aquaporin inhibitor, mercuric chloride. The control (held at 4°C for 4 h) was incubated in Coast's solution + 0.25 mol l^–1 glycerol. The remaining groups, labelled Coast's, Coast's + glycerol (Coast's solution + 0.25 mol l^–1 glycerol) and mercuric chloride (Coast's solution + 0.25 mol l^–1 glycerol + 0.2 mmol l^–1 HgCl₂), were cooled from 4°C to –20°C over 2 h and held at –20°C for 2 h before cell viability was assessed (N=4 per treatment). Different letters signify a significant difference between mean cell survival among treatments (P<0.05).