Fig. 2. Immunoidentification of (A) AQP2 (N=2), (B) AQP3 (N=3) and (C) AQP4 (N=2) in protein extracts of E. solidaginis larvae using mammalian antisera. Fifty micrograms of protein were loaded onto each lane. Larvae were acclimated for 4 months at 4°C or –20°C. Desiccated larvae were kept at 0% relative humidity for 4 days. Below each band on these representative gels is the corresponding average relative abundance (RA), with the RA of 4°C treated samples set to 1.0.