Fig. 4. Vma2p^Arch-containing yeast show increased sensitivity to cycloheximide and wortmannin. VMA2 yeast cells carrying no plasmid (Null) or transformed with yeast B subunit (WT), Vma2p^Phe (Phe) or Vma2p^Arch (Arch) plasmids were grown to log phase, carefully counted so that each type of cell was at the same starting concentration, subjected to serial 10-fold dilutions, and then transferred to plates with control media (A) or media containing sub-lethal doses of various drugs (B) as indicated in Materials and methods for 3 days. Ct, camptothecin; Hu, hydroxyurea; Cx, cycloheximide; Wt, wortmannin; Sm, sulfometuron methyl; Fk, FK506.