Fig. 8. Glucose (A), glycogen (B) and lactate (C) levels, and glucokinase (D)
activities in Brockmann bodies of rainbow trout incubated in vitro
for 1 h at 15°C in modified Hanks' medium containing 2, 4 or 8 mmol
l–1 L-(+)-lactate alone (control) or with 50 mmol
l–1 sodium oxamate, 1 mmol l–1
4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid disodium salt
hydrate (DIDS), 1 mmol l–1 sodium dichloroacetate (DCA), 10
mmol l–1 2-deoxy-D-glucose (2-DG), 1 mmol
l–1
-cyano-4-hydroxy cinnamate (4-CIN) or 10 mmol
l–1 D-glucose. Each value is the mean ±
s.e.m. of ten (control) or three (treatments) independent experiments carried
out with pools of tissues from three to four different fish (21 pools per
experiment: seven treatments times three glucose concentrations). Different
letters indicate significant differences (P<0.05) among treatments
within each lactate concentration; #significantly different from
groups incubated with 2 mmol l–1 lactate at the same
treatment (P<0.05);
significantly different
from groups incubated with 4 mmol l–1 lactate at the same
treatment (P<0.05).