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Figure 7


Fig. 7. Glucose (A), glycogen (B) and lactate (C) levels, and glucokinase (D) and lactate dehydrogenase reductase (E) activities in hindbrain of rainbow trout incubated in vitro for 1 h at 15°C in modified Hanks' medium containing 2, 4 or 8 mmol l–1 L-(+)-lactate alone (control) or with 50 mmol l–1 sodium oxamate, 1 mmol l–1 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid disodium salt hydrate (DIDS), 1 mmol l–1 sodium dichloroacetate (DCA), 10 mmol l–1 2-deoxy-D-glucose (2-DG), 1 mmol l–1 {alpha}-cyano-4-hydroxy cinnamate (4-CIN) or 10 mmol l–1 D-glucose. Each value is the mean ± s.e.m. of ten (control) or three (treatments) independent experiments carried out with pools of tissues from three to four different fish (21 pools per experiment: seven treatments times three glucose concentrations). Different letters indicate significant differences (P<0.05) among treatments within each lactate concentration. #Significantly different from groups incubated with 2 mmol l–1 lactate at the same treatment (P<0.05); {dagger}significantly different from groups incubated with 4 mmol l–1 lactate at the same treatment (P<0.05).