JEB -- Reis-Santos et al. 211 (6): 978 Figure IG2

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Fig. 2. Branchial Na⁺/K⁺-ATPase activity (µmol ADP mg^–1 protein h^–1) (A,B) and ${alpha}$ subunit expression determined by immunoblotting (C,D) in ammocoetes (white bars) and transformers (grey bars) of P. marinus. (A,C) Ammocoetes and transformers (stages 3–5) acclimated to freshwater (FW) and 25 ${per thousand}$ salinity and sampled from the Fort River, MA, USA (hatched bars). (B,D) Ammocoetes and transformers (stage $>=$ 6) acclimated to deionized water (DW), FW or saline water of 10, 20, 30 and 35 ${per thousand}$ . Representative immunoblots of sea lamprey ammocoete and transformer gill probed with the ${alpha}$ subunit antibody ${alpha}$ RbNKA are shown above their respective graphs. Bars with like characters are not significantly different (ANOVA; P<0.05). Upper and lower case characters are used for transformers and ammocoetes, respectively, in B and D. Asterisks indicate a significant difference from the ammocoetes at a given salinity.