Fig. 2. Western blots of mosquito larval membrane proteins separated on SDS–PAGE and probed with a polyclonal antibody to subunit B of the H⁺ V-ATPase (A), a monoclonal antibody to subunit of the Na⁺/K⁺ P-ATPase (B), and polyclonal epitope-specific antibodies to AgNHA1 (C) and AgNAT8 (D). The arrows indicate the band of protein that is recognized by each antibody. The nitrocellulose membrane was cut into strips and probed with the different antibodies leading to differences in the backgrounds of the blots.