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Figure 8


Fig. 8. Relative levels of histone H3 (A) and H4 (B) determined by ChIP assay. The monocytes were treated with 100 µmol l–1 Hcy, folate, AZC and TSA for 24, 48 and 72 h. Nuclear extracts were immunoprecipitated with antibody against AcH3 and AcH4, and relative levels of histones H3 and H4 were analyzed by SDS–PAGE. *P<0.05, compared with positive group; {dagger}P<0.05, compared with 100 µmol l–1 Hcy group (72 h).