Fig. 6. Effects of Hcy on DNMT1, DNMT3, MBD2, MeCP2 protein levels in human monocytes incubated for different times (A) and with different antagonists (B). The monocytes were incubated in the absence or presence of Hcy at various concentrations and immunoblots (top) analyzed by densitometry (below). The results were expressed as percentage of control (100%) ± s.e.m., each performed in duplicate. Relative value was related to that of β-actin through the formula (relative value=experiment densitometry valuex100/β-actin value).