Fig. 4. Relative EC-SOD protein (A) and mRNA (B) levels. Monocytes were treated with 100 µmol l^–1 Hcy in control medium for 24, 48 or 72 h. Total RNA was isolated and real-time PCR was carried out to detect mRNA levels. Protein levels of EC-SOD (A) were examined by western blotting (B). Values are means ± s.e.m. from 3 separate experiments with triplicated samples (N=6).^*P<0.05, compared with positive group; P<0.05, compared with 100 µmol l^–1 Hcy group (72 h).