Fig. 5. Expression of White in the Drosophila Malpighian tubule. Staining of White expression in c42/w::eYFP adult tubules by immunocytochemistry with anti-GFP antibody. Intact tubules from adult progeny of crosses between c42 and two independently derived UAS-w::eYFP lines (E5 and H8) were used. (A) Main segment of c42/w::eYFP (UAS line E5) tubule showing exclusion of staining in stellate cells, yellow arrows. (B) Main segment of c42/w::eYFP (UAS line H8) showing exclusion of staining in stellate cells, yellow arrows. (C) Canton S tubules (high magnification) stained with anti-GFP antibody. Only background fluorescence is observed. (D) Principal cell in c42/w::eYFP (UAS line E5) tubule at high magnification showing vesicular staining. Scale bars: A,B 10 µm; C,D 5 µm.