Fig. 3. Light micrographs of 5 µm thick sections showing TonEBP immunoreactivity (TonEBP-IR) in the renal cortex (A,B) and outer medulla (C,D) of control (A,C) and 14-day water-deprived N. alexis (B,D). In A and B, black arrowheads indicate the glomeruli. TonEBP-IR is weak in the renal cortex and is evenly distributed between the cytoplasm and nucleus of control (A) and 14-day water-deprived mice (B). In C and D, black arrows indicate the nuclei of epithelial cells lining the tubules that pass through the outer medulla, and arrowheads indicate the tubule lumen. Moderate TonEBP-IR can be observed in the nuclei and cytoplasm in the epithelial cells lining the renal tubules of control N. alexis (C), but TonEBP-IR was more intense in the nuclei than the cytoplasm of the epithelial cells in the outer medulla of 14-day water-deprived mice (D). Scale bars, 30 µm (A,B), 120 µm (C,D).