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Figure 6


Fig. 6. Human insulin stimulated extracellular signal-regulated kinase (ERK, A) and Akt/protein kinase B (PKB, B) phosphorylation in the A. stephensi midgut. Midgut proteins prepared from A. stephensi at 30 min after feeding on artificial bloodmeal supplemented with human insulin were probed with anti-phospho-ERK antisera (p-Erk, 1:10 000; A) or with anti-phospho-Akt antibody (p-Akt, 1:1000; B). Anti-total ERK (ERK) or anti-total Akt/PKB (Akt) antibody was used to assess loading.