Fig. 3. Example survivorship curve showing that human insulin provided by
artificial bloodmeal increased the mortality of A. stephensi relative
to controls and that this effect was reversed by the provision of the
cell-permeable SOD mimetic agent MnTBAP in the presence of insulin. This
example corresponds to experiment 5, Table
2. Female mosquitoes were provided with
1.7x10–3 µmol l–1 human insulin,
0.05 mmol l–1 MnTBAP alone (not shown), human insulin +
MnTBAP, or with an equivalent volume of insulin buffer by artificial bloodmeal
every Monday, Wednesday and Friday until all insects were dead. Oviposition
cups and 10% sucrose pads were provided between bloodmeals. Dead insects were
counted and removed daily from treatment and control cartons.