Fig. 3. S2 cell viability following Cu limitation. S2 cells were pre-treated with Mvl or control dsRNA for 6 days. Real-time PCR demonstrated Mvl was suppressed to 47.3±16.1% (mean ± s.e.m.) of control after 6 days. Cells were exposed to basal medium or Cu-limited medium using 10 µmol l^–1 Diamsar for 6 days and viable cells were counted. Values are mean ± s.e.m. of nine replicates from three independent experiments. An independent samples t-test was used to determine significant differences. ^*P<0.05 compared to basal medium, P<0.05 compared to control cells exposed to Cu-limited medium.