Fig. 2. Cu accumulation in S2 cells. Cu accumulation was measured in S2 cells exposed to 2 µmol l^–1 Cu. Intracellular levels were measured after 1 h and 24 h and normalised to total cellular protein. An independent samples t-test was used to determine significant differences. (A) Cu accumulation in S2 cells stably overexpressing Mvl, expressed relative to an empty vector control. Values are mean ± s.e.m. of 15 replicates from four independent experiments. ^*P<0.0005 compared to control. (B) Cu accumulation following dsRNAi knockdown of Mvl, expressed relative to control. Cells were pre-treated with Mvl or control dsRNA 48 h prior to Cu exposure. Real-time PCR demonstrated Mvl expression was reduced to 18.1±4.6% (mean ± s.e.m.) of control after 48 h. Values are mean ± s.e.m. of 21 replicates from seven independent experiments. P<0.0001 compared to control.