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Figure 1


Fig. 1. Mvl expression and localisation in S2 cells. (A) Western blotting using anti-FLAG antibody detected a 52 kDa band corresponding to Mvl in lysate from pAcMvl cells. No significant signal was visible in lysate from empty vector control cells (pAc). {alpha}-Tubulin was used as a loading control. (B,C) Mvl was detected in S2 cells by immunofluorescence using anti-FLAG and Alexa Fluor 488 anti-mouse antibodies (green) and the nucleus was stained with DAPI (blue). Mvl was detected at the plasma membrane as well as in punctate structures in pAcMvl cells (B), but no significant staining was visible in pAc cells (C).