Fig. 8. Western blot detection of cytochrome c protein in mitochondrial and cytosolic fractions of euthyroid and hypothyroid rat Harderian gland (HG). In the euthyroid HG, the intensity of the cytochrome c band (14 kDa) in the mitochondrial pellet was significantly (P<0.01) higher than that in cytosolic fraction. Conversely, in hypothyroid HG, the cytochrome c band was significantly more intense (P<0.01) in the cytosolic fraction than in the mitochondrial pellet. Each mitochondrial and cytosolic preparations contained three glands from three different rats. C, cytosolic fraction; M, mitochondrial pellet.