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Figure 5


Fig. 5. Effects of cadmium (Cd) exposure on expression of metallothionein mRNA in gill and hepatopancreas (HP) cells of oysters. Metallothionein levels were measured using real-time PCR, normalized to the actin mRNA levels and expressed as % of the respective controls. Cells were exposed to varying Cd levels (x-axis) for 4 h at 20°C. (A) mRNA for total metallothioneins and (B) for metallothionein I only. Vertical bars represent s.e.m. Asterisks denote values significantly different from the respective controls (P<0.05). N=9.