JEB -- Hille and Walz 211 (4): 568 Figure IG3

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Fig. 3. Effects of various factors on intracellular acidification induced by 1 µmol l^–1 DA in duct cells of lobe preparations in HCO₃^–-free saline. (A) The acidification is abolished in Cl^––free saline (N=4). (B) The acidification is not influenced by bath application of 500 µmol l^–1 DIDS (N=9). (C) The acidification is transient following the bath application of 10 µmol l^–1 bumetanide (N=6). (D) The combined bath application of 10 µmol l^–1 bumetanide and 500 µmol l^–1 DIDS abolishes the acidification (N=6). (E) The acidification is not influenced by bath application of 500 µmol l^–1 acetozolamide (N=4). (F) The combined bath application of 10 µmol l^–1 bumetanide and 500 µmol l^–1 acetazolamide reduces the acidification dramatically (N=8).