Fig. 3. Maternal transfer of hydrocarbons to oocytes and the fate of radiolabeled hydrocarbons in embryos and first- and second-instar nymphs. Vitellogenic day-3 females were injected with [1-¹⁴C]propionate (A) or [³H]3,11-dimethylnonacosane (B), and radiolabeled hydrocarbons were then extracted, purified and quantified on the external surface and internal tissues of the mothers, egg cases (and embryos) and nymphs. Data are normalized to show percentages of the total radiolabeled hydrocarbons in the mother and her offspring. Data are means ± s.e.m. (N=5–8). Significant differences among means within a series are represented by different letters (ANOVA, LSD, P<0.05) and between internal and external hydrocarbons of nymphs by asterisks (paired t-test, P<0.05).