Fig. 4. The effect of preloading flying crickets with trehalose on: (A) total lipid in hemolymph of flying cricket preloaded with trehalose (Tre Fly) and of flying cricket preloaded with insect Ringer (R Fly) N=12, control (Con) N=10; (B) relative amount of free apoLpIII, measured as the band darkness ratio (see Materials and methods) N=4/group; (C) resistance to infection with S. marcescens, R Con N=35, Tre Con N=37, Tre Fly N=39, R Fly, N=39. Bars are means with 95% CI in A and B. Con, non-flying control; R Con, non-flying control preloaded with insect Ringer; Tre Con, non-flying cricket injected with trehalose. NS, not significantly different. In B and C there is a significant decreasing trend across groups (see text).