Fig. 5. Reactive oxygen species production depends on the activation status of endoneurial phagocytes. (Ai and Aii, left panels) DIC images of a spreading endoneurial phagocyte (i) and non-spreading endoneurial phagocyte (ii). (Ai and Aii, right panels) Epifluorescence images showing CM-H₂DCF fluorescence (i), or the absence thereof (ii), in the same cells as shown in the corresponding left panels. Note that the CM-H₂DCF fluorescence is contained inside the activated endoneurial phagocyte. (B) Percentage of CM-H₂DCF fluorescence-positive endoneurial phagocytes when cultured in ABS only, ABS + 1 µmol l^–1 cGRGDSPA and ABS + 100 µmol l^–1 cGRGDSPA. Note that independent of culture condition, the majority of the spreading cells are CM-H₂DCF fluorescence positive while only a very small percentage (<3%) of the non-spreading cells display CM-H₂DCF fluorescence. Image acquisition conditions were exactly the same for all cells. Scale bar in A, 10 µm.